EBV DNA, PCR Tips

Breast cancer begins when cells in the breast start to get out of control. These cells are mostly in the form of a tumor that can be regularly seen on an x-ray or felt as a lump. The tumor is dangerous (malignant) if the cells attack the surrounding tissues or spread to far off zones in the body. Breast cancer happens mostly in women, yet men can get it, as well.

This is how breast cancer can spread:

• Bosom cancer can spread through the lymph framework
• The lymph framework includes lymph nodes, lymph vessels, and lymph liquid found all throughout the body
• Lymph nodes are little, bean-shaped accumulations of immune system cells that are connected by lymph (or lymphatic) vessels. Lymph vessels resemble little veins, with the exception of that they transport a liquid called lymph (rather than blood) far from the breast
• Lymph contains tissue liquid, waste items, and immune system cells. Breast cancer cells can enter lymph vessels and start to develop into lymph nodes

A large portion of the lymph vessels of the breast deplete into the:

• Lymph nodes under the arm (axillary hubs)
• Lymph nodes around the neck bone (supraclavicular and infraclavicular lymph hubs)
• Lymph nodes inside the neck close to the breast bone (inner mammary lymph hubs)

Following are some of the causes of breast cancer:

• Hormones play a part in creating breast cancer disease; however exactly how this happens is not completely known
• Ordinary breast cells get to be distinctly carcinogenic in view of changes (transformations) in DNA
• Yet, most DNA changes identified with breast cancer are gained in breast cells during a woman’s life as opposed to having been acquired
• Qualities that accelerate cell division are called oncogenes
• Tumor silencer qualities are typical qualities that back off cell division, repair DNA oversights, or advise cells when to bite the dust
• Certain acquired DNA transformations (changes) can drastically increase chances for building up specific growths and are in charge of a large number of tumors that keep running in few families

Some of the treatments of breast cancer include:

• Health alterations: Body weight, physical action, and eating routine are all connected to breast tumor, so these may be territories where you can make a move.
• Restorative alternatives for women at expanded hazard: For women who have certain hazard components for breast growth, for example, a family history; various restorative alternatives may counteract breast cancer.
• Medications to lessen hazard: For women at expanded danger of breast cancer, medications, for example, tamoxifen and raloxifene, can reduce the hazard. However, these medications can have their own dangers and symptoms.
• Preventive surgery: In case that you have a solid family history of breast cancer, you can talk with your specialist about hereditary testing. This test addresses changes in qualities that increase the danger of breast cancer; for example, the BRCA qualities. In case you have a hereditary change from a family with a transformation, however, have not been tried, you could consider surgery to bring down your danger of tumor.

HIV: QUICK FACTSIntroductionThe human immunodeficiency virus (HIV) is a subgroup of retrovirus (lentivirus) that causes HIV infection. After a period of time, this infection leads to what is called acquired immunodeficiency syndrome (AIDS).

Epidemiology

According to the Centers for Disease Control and Prevention (CDC), from 2010-2015, the estimated rate of HIV infection in all 50 US states decreased from 14.2 per 100,000 population in 2010 to 12.3 per 100,000 population in 2015. In 2015, 39,513 individuals were diagnosed with HIV infection. From 2010 to 2014, the annual number of new HIV infections decreased by 9%.

Virology HIV-1 and HIV-2 are retroviruses in the Retroviridae family, Lentivirus genus. They are enveloped, diploid, single-stranded RNA viruses with a DNA intermediate.Upon entry into the target cell, the viral RNA genome is converted into double-stranded DNA by an enzyme, reverse transcriptase that is transported along with the viral genome in the virus particle. The resulting viral DNA is then imported into the cell nucleus and integrated into the cellular DNA by a virally encoded enzyme, integrase. HIV contains three retroviral genes, namely gag, pol, and env.

• Gag gene: Encodes group-specific antigen; the inner structural proteins
• Pol gene: Encodes polymerase; it also contains integrase and protease
• Env gene: Encodes the viral envelope, the outer structural proteins responsible for cell-type specificity. Glycoprotein 120, the viral envelope protein, binds to the host CD4+molecule

Pathophysiology

HIV infects vital cells in the human immune system, such as helper T cells (specifically CD4+ T cells), macrophages, and dendritic cells and leads to cellular immune deficiency. This results in the development of opportunistic infections and neoplastic processes.

Mode of Transmission
Sexual intercourse: Unprotected sexual intercourse; especially receptive anal intercourse and different sexual partnersContact with or transfer of blood, pre-ejaculate, semen, and vaginal fluids infected with the virus.Spread from an infected mother to her infant through breast milk. An HIV-positive mother can transmit HIV to her baby both during pregnancy and childbirth due to exposure to her blood or vaginal fluid.

Signs and Symptoms There are 3 categories of infection

• Category A: Asymptomatic HIV infection without a history of symptoms or AIDS-defining conditions
• Category B: HIV infection with symptoms that are directly attributable to HIV infection
• Category C: HIV infection with AIDS-defining opportunistic infections

Investigation

A high-sensitivity enzyme-linked immunosorbent assay (ELISA) should be used for screening.

Management

• Start with antiretroviral therapy (ART) in all HIV-infected adults ready to start therapy.
• CD4 count is no longer a criterion for starting the therapy.
• Use of nucleoside reverse transcriptase inhibitors and protease inhibitors.
• For refractory cases, during the failing treatment regimen and before switching therapy, use rapid confirmation, perform resistance testing and reevaluate.

In case you have a concern or query you can always consult an expert & get answers to your questions!

Breast cancer begins when cells in the breast start to get out of control. These cells are mostly in the form of a tumor that can be regularly seen on an x-ray or felt as a lump. The tumor is dangerous (malignant) if the cells attack the surrounding tissues or spread to far off zones in the body. Breast cancer happens mostly in women, yet men can get it, as well.

This is how breast cancer can spread:

• Bosom cancer can spread through the lymph framework
• The lymph framework includes lymph nodes, lymph vessels, and lymph liquid found all throughout the body
• Lymph nodes are little, bean-shaped accumulations of immune system cells that are connected by lymph (or lymphatic) vessels. Lymph vessels resemble little veins, with the exception of that they transport a liquid called lymph (rather than blood) far from the breast
• Lymph contains tissue liquid, waste items, and immune system cells. Breast cancer cells can enter lymph vessels and start to develop into lymph nodes

A large portion of the lymph vessels of the breast deplete into the:

• Lymph nodes under the arm (axillary hubs)
• Lymph nodes around the neck bone (supraclavicular and infraclavicular lymph hubs)
• Lymph nodes inside the neck close to the breast bone (inner mammary lymph hubs)

Following are some of the causes of breast cancer:

• Hormones play a part in creating breast cancer disease; however exactly how this happens is not completely known
• Ordinary breast cells get to be distinctly carcinogenic in view of changes (transformations) in DNA
• Yet, most DNA changes identified with breast cancer are gained in breast cells during a woman’s life as opposed to having been acquired
• Qualities that accelerate cell division are called oncogenes
• Tumor silencer qualities are typical qualities that back off cell division, repair DNA oversights, or advise cells when to bite the dust
• Certain acquired DNA transformations (changes) can drastically increase chances for building up specific growths and are in charge of a large number of tumors that keep running in few families

Some of the treatments of breast cancer include:

• Health alterations: Body weight, physical action, and eating routine are all connected to breast tumor, so these may be territories where you can make a move.
• Restorative alternatives for women at expanded hazard: For women who have certain hazard components for breast growth, for example, a family history; various restorative alternatives may counteract breast cancer.
• Medications to lessen hazard: For women at expanded danger of breast cancer, medications, for example, tamoxifen and raloxifene, can reduce the hazard. However, these medications can have their own dangers and symptoms.
• Preventive surgery: In case that you have a solid family history of breast cancer, you can talk with your specialist about hereditary testing. This test addresses changes in qualities that increase the danger of breast cancer; for example, the BRCA qualities. In case you have a hereditary change from a family with a transformation, however, have not been tried, you could consider surgery to bring down your danger of tumor.

In case you have a concern or query you can always consult an expert & get answers to your questions!

Today, prenatal testing can help pinpoint your unborn child’s risk to a number of genetic conditions. NIPT or non-invasive prenatal testing is a modern form of prenatal testing that does not harm the mother or child. NIPT involves a simple blood test that can be performed as an outpatient procedure in any doctor’s office. Blood for this test is collected from a vein in the mother’s arm. The DNA from this blood sample is then studied in a laboratory to look for signs of abnormalities. These results are them compared to your first-trimester ultrasound results to determine if further testing is required.

There are a number of advantages to this form of testing

1. Highly Accurate: NIPT results are said to be accurate in 99% cases. This clears doubts about chromosomal abnormalities and helps parents to be dealing with problems in their unborn children. For example, NIPT testing can be used to diagnose problems like Down syndrome. NIPT also decreases the need for other forms of invasive prenatal testing.
2. Safe: NIPT is noninvasive and does not harm the health of the baby or the mother. Since it involves only a simple blood test, it does not cause pain or discomfort either. It also has no connection to miscarriages. Pregnant woman above the age of 35 and those who have had an ultrasound showing an increased risk of aneuploidy or those who have a history of trisomy can benefit from this form of pre-natal testing. The only known alternative to this form of testing involves withdrawing DNA samples from the amniotic sac with the help of a needle. This form of testing can result in a miscarriage in rare cases.
3. Comfortable: Unlike prenatal tests that require precision while withdrawing DNA from the amniotic sac, the NIPT requires far less expertise. This makes women much more comfortable undergoing this form of testing as compared to the other.
4. Early Diagnosis: With prenatal testing parents do not have to wait till a baby is born to find out if he or she suffers from any genetic complications. NIPT can be scheduled as early as 10 weeks into the pregnancy and hence gives the parents enough time to prepare themselves to deal with the issue if any.

However, you should remember that NIPT cannot be used to replace diagnostic tests such as amniocentesis. Also, it does not detect all chromosomal abnormalities.

While some couples conceive with ease, conceiving a child can be extremely difficult for others. In cases where a woman is not able to get pregnant despite having regular intercourse in tune with her biological cycle, infertility tests may be suggested to investigate the reason behind this. In most cases, these tests are suggested if a year has gone by without intercourse resulting in a pregnancy. Women may also be said to be infertile, if they cannot carry a foetus to full term.

Infertility can affect both men and women and can be triggered by a number of different reasons. In some cases, it is treatable while in others, alternative ways of having a family may need to be discussed. Hence, it is very important to understand the different types and triggers for infertility and to undergo complete infertility investigations. Some of the common types of infertility investigations are as given below.

1. Blood tests: A blood test of both partners is used to assess the general health of the couple and to identify any health factors that may be impeding fertility.
2. Tests for women: 
   1. Hysterosalpingogram: This is an X ray that can identify if a blockage or any other issue in the fallopian tubes are the cause for infertility.
   2. Pelvic ultrasound: This is used to have a look at the anatomy of the female reproductive system and to check for fibroids.
   3. Laparoscopy / hysteroscopy: This is used to get a clearer picture of the ovaries, uterus and other parts of the reproductive system.
   4. Tracking cycle: Ovulation and the thickness of the uterus lining may also affect infertility, This can be diagnosed by tracking and assessing the menstrual cycle.
3. Tests for men
   1. Semen analysis:This test is used to get an idea of sperm concentration, motility and the appearance of sperm in a man’s semen. In some cases, the semen may not include any sperm cells. In such cases, a follow up investigation may be required to assess if sperm cells are being created in the testicles. This is known as testicular sperm aspiration or a testicular biopsy.
   2. Sperm DNA: The DNA in sperm also plays an important role in fertility. This test is used to assess the sperm DNA integrity and to see how it may affect embryo development and chances of an on-going pregnancy.

Infertility treatment depends on the cause identified by the above tests and hence it is important for both partners to undergo complete testing.

In case you have a concern or query you can always consult an expert & get answers to your questions!

While some couples conceive with ease, conceiving a child can be extremely difficult for others. In cases where a woman is not able to get pregnant despite having regular intercourse in tune with her biological cycle, infertility tests may be suggested to investigate the reason behind this. In most cases, these tests are suggested if a year has gone by without intercourse resulting in a pregnancy. Women may also be said to be infertile, if they cannot carry a foetus to full term.

Infertility can affect both men and women and can be triggered by a number of different reasons. In some cases, it is treatable while in others, alternative ways of having a family may need to be discussed. Hence, it is very important to understand the different types and triggers for infertility and to undergo complete infertility investigations. Some of the common types of infertility investigations are as given below.

1. Blood tests: A blood test of both partners is used to assess the general health of the couple and to identify any health factors that may be impeding fertility.
2. Tests for women: 
   1. Hysterosalpingogram: This is an X ray that can identify if a blockage or any other issue in the fallopian tubes are the cause for infertility.
   2. Pelvic ultrasound: This is used to have a look at the anatomy of the female reproductive system and to check for fibroids.
   3. Laparoscopy / hysteroscopy: This is used to get a clearer picture of the ovaries, uterus and other parts of the reproductive system.
   4. Tracking cycle: Ovulation and the thickness of the uterus lining may also affect infertility, This can be diagnosed by tracking and assessing the menstrual cycle.
3. Tests for men
   1. Semen analysis:This test is used to get an idea of sperm concentration, motility and the appearance of sperm in a man’s semen. In some cases, the semen may not include any sperm cells. In such cases, a follow up investigation may be required to assess if sperm cells are being created in the testicles. This is known as testicular sperm aspiration or a testicular biopsy.
   2. Sperm DNA: The DNA in sperm also plays an important role in fertility. This test is used to assess the sperm DNA integrity and to see how it may affect embryo development and chances of an on-going pregnancy.

Infertility treatment depends on the cause identified by the above tests and hence it is important for both partners to undergo complete testing.

Conventional wisdom makes us believe that the only type of cancer caused due to smoking is lung cancer. However, recent studies have greatly detailed the fact that smoking or the consumption of tobacco products can cause esophagus, pancreas, liver, kidney, stomach, among other types of cancer.

The Various Chemical Carcinogens Present In Tobacco
Thus, smoking is by far the largest preventable cause of cancer. The solution is simple: stop smoking. Tobacco products contain certain elements that are widely believed to cause cancer. These elements also referred to as carcinogens are either naturally part of the tobacco-producing plant or are added to it by the tobacco manufacturer. The latter carcinogens include benzene, certain tobacco-specific nitrosamines, and formaldehyde. The quantity and the specificity of carcinogens differ from manufacturer to manufacturer and country to country.

Continuous consumption of products which contain carcinogens will inevitably result in the formation of cancer. The IARC (International Agency for Research on Cancer) has already designated certain chemicals found in tobacco and tobacco-related products as human carcinogens.

The Effects of the Chemical Carcinogens -
It must be emphasized that there is no safe way to consume tobacco. From cigarettes and pipes to smokeless tobacco and cigars – all of them lead to the same result. Chemicals found in tobacco reduce the ability of our immune system, making it harder for it to kill cancerous cells. This ensures that cancerous cells simply multiply without it being stopped or killed. Moreover, the same chemicals also alter the “DNA structure” and the instructions of the DNA which leads to cells growing out of control and abetting the cancerous tumor.

How do the carcinogens enter our bloodstream?
These chemicals are delivered into our bloodstream by virtue of us breathing in the smoke. Once the chemicals enter our bloodstream, it travels to all parts of our body – carrying this carcinogenic chemical. Non-smokers who stand beside individuals who are smoking (secondhand smoking) become passive smokers, also inhale the harmful smoke. The World Health Organization estimates that 10% of all smoking-related deaths are due to passive or secondhand smoking.

How long does it take for cancer to develop?
While there is no specific time-frame linking consumption of tobacco to the development of cancer, it is estimated that it takes many years or even decades for cancer to develop. Our body’s immune system is vested with the power to cope up with limited damage, however, persistent smoking tendencies and the chemical carcinogens entering our bloodstreams – irreversibly damage the DNA in many lung cells.

Thus, in conclusion, it is highly advisable to quit smoking sooner rather than later. Tobacco causes long-term and irreparable damage to our bodily organs.

With the evolution of medical technology, we have made inroads when it comes to evolved methods of treating a variety of ailments and conditions. The use of lasers is now commonplace for a number of surgeries and non-invasive procedures that can help in treating many conditions. From hair removal to surgeries, there are a number of places where lasers enjoy medical and cosmetic use. There is a common misnomer that lasers have detrimental effects and may even be carcinogenic or cancer causing in nature.

Let us find out why lasers cannot cause cancer.

1. Penetration of light: For many people, it may be a cause of concern that laser treatment of the hair, skin or other parts of the body is done with the penetration of high energy beams in that part of the body. Yet, the beams do not penetrate that deeply. Usually, this penetration only happens to the extent of hair follicles and skin, which leaves the internal organs safe.
2. Redness: At the very maximum, the lasers can cause a reaction like redness on the skin. This does not mean that the lasers are capable of causing cancer. This can simply be treated with the help of ice application on the affected area.
3. Mutations: There have been many reports that say the ionising radiation of the laser beams can cause DNA mutations. Yet, this is not true. These DNA mutations can lead to the manifestation and production of abnormal and cancerous cells, which can further progress and spread to become malignant tumours. Yet, laser treatments and applications do not have the power to cause such DNA mutations, and there is no medical evidence that suggests the same.
4. Treating areas near reproductive organs: There are many women who opt for laser hair removal which is a more permanent solution for waxing and other forms of hair removal that we go through on a regular basis. But many women are also concerned that it may actually cause problems and cancer when it comes to the reproductive organs, in case the hair removal treatment and lasers are applied to the area near these organs. This is not true because the laser beams are merely applied to the hair follicles of the area. Since, laser beams are not applied to the reproductive organ, which ensures that there is no effect on the internal reproductive organ in any way.

Before trying out laser treatment, it is important to try it on a small portion of your skin so that you can be sure that it does not affect you and cause a reaction. Further, for laser related surgeries, it is important to turn to a well known clinic, hospital or doctor so that the right level of energy is used during the surgery.  In case you have a concern or query you can always consult an expert & get answers to your questions!

While some couples conceive with ease, conceiving a child can be extremely difficult for others. In cases where a woman is not able to get pregnant despite having regular intercourse in tune with her biological cycle, infertility tests may be suggested to investigate the reason behind this. In most cases, these tests are suggested if a year has gone by without intercourse resulting in a pregnancy. Women may also be said to be infertile, if they cannot carry a foetus to full term.

Infertility can affect both men and women and can be triggered by a number of different reasons. In some cases, it is treatable while in others, alternative ways of having a family may need to be discussed. Hence, it is very important to understand the different types and triggers for infertility and to undergo complete infertility investigations. Some of the common types of infertility investigations are as given below. 

1. Blood tests: A blood test of both partners is used to assess the general health of the couple and to identify any health factors that may be impeding fertility.
2. Tests for women: 
   1. Hysterosalpingogram: This is an X ray that can identify if a blockage or any other issue in the fallopian tubes are the cause for infertility.
   2. Pelvic ultrasound: This is used to have a look at the anatomy of the female reproductive system and to check for fibroids.
   3. Laparoscopy / hysteroscopy: This is used to get a clearer picture of the ovaries, uterus and other parts of the reproductive system.
   4. Tracking cycle: Ovulation and the thickness of the uterus lining may also affect infertility, This can be diagnosed by tracking and assessing the menstrual cycle.
3. Tests for men
   1. Semen analysis:This test is used to get an idea of sperm concentration, motility and the appearance of sperm in a man’s semen. In some cases, the semen may not include any sperm cells. In such cases, a follow up investigation may be required to assess if sperm cells are being created in the testicles. This is known as testicular sperm aspiration or a testicular biopsy.
   2. Sperm DNA: The DNA in sperm also plays an important role in fertility. This test is used to assess the sperm DNA integrity and to see how it may affect embryo development and chances of an on-going pregnancy.

Infertility treatment depends on the cause identified by the above tests and hence it is important for both partners to undergo complete testing. In case you have a concern or query you can always consult an expert & get answers to your questions!

It is as important for the man as it is for the woman to undergo certain tests before the IVF cycle is started. Fertility is not only an issue for a woman, but it also might be for the man in question. There are certain tests that need to be conducted on men before they can gear up for IVF:

1. Semen Analysis: One of the most important tests for male infertility is semen analysis. Sometimes, a man may be completely healthy, but his sperm production could be of poor quality. Semen analysis is done to provide an accurate evaluation of sperm number, the ability to move (motility), size and shape (morphology), as well as the consistency and volume of the sample collected. Semen analysis (SA) test includes only the sperm count. Sperm Funtion test (SFT) will include in detail on morphology, motility and other parameters of the sperm.

2. Trial Wash: This is also known as sperm wash. It is mostly done to remove chemicals from the sperm. Not doing a sperm wash might end up affecting the later stages of the IVF treatment. In this process, the misshapen and immotile sperm is separated so a clean sample of motile and healthy sperm can be collected.

3. TUNEL Assay: Since sperms build up about half of a child's genetic makeup, a TUNEL Assay or 'sperm DNA test' is required. This test is done to measure the sperm population that have fragmented DNA. Negative impacts on genetics can hamper the success of the fertility treatment.

DNA fragmentation index (DFI) test is usually recommended to men with:

• Leukocytes detected by the sperm analysis
• Age approaching 50
• A history of diabetes II type or prostatitis
• Exposure to chemicals or prolonged heat at workplaces

4. Screening for Infectious Diseases: This test is done for both the partners to check for diseases like AIDS, Hepatitis C, and so on. In case you have a concern or query you can always consult an expert & get answers to your questions!