Question

This is my moms report please I am confused give me solution the test says vivid is negative but it also says some kind of sabicovirus detected what is the conclusion!
molecular biology
covid-19 rt pcr (qualitative)
primary sample type: nasopharyngeal swab
e gene (b-βcov)
(rt pcr)
not detected
rdrpgene and n gene (sars-cov-2)
(rt pcr)
not detected
ct value (rdrpgene and n gene)
(rt pcr)
not applicable
conclusion
(rt pcr)
sample is negative for sars-cov-2 rna
remarks. Icmr registration no: rpplpmp
the test has been performed at (polo labs pvt. Ltd. Ivy hospital, sector 71, mohali).
Clinical utility of the test
coronaviruses (cov) are a large family of viruses that cause illness ranging from the common cold to more severe diseases such as middle east
respiratory syndrome (mers-cov) and severe acute respiratory syndrome (sars-cov). Sars-cov-2, is the causative agent of the coronavirus disease 2019
(covid-19). Common signs of infection include respiratory symptoms, fever, cough, shortness of breath and breathing difficulties. In more severe cases,
infection can cause pneumonia, severe acute respiratory syndrome and organ failure. The sars-cov-2 rna is generally detectable in nasopharyngeal /
oropharyngeal swabs during the acute phase of infection. Positive results are indicative of active infection. This assay is based on real-time pcr technology, for
the qualitative detection of cavity-19 viral rna. This real time pcr assay targets both the e-gene (b-βcov) which is recommended as a screening gene to
detect the presence of lineage b-betacoronovirus and rdrp/n gene (sars-cov-2), which confirms presence of infection with covid-19.
Note: this test is performed using icmr approved kit.
Interpretation:
sr. No. Result comment
1
both e gene and rdrp and n gene not
detected
absence of e gene and sars-cov-2 rna indicate no infection with
sarbecovirus (b-βcov).
2
e gene detected and rdrp and n gene
not detected
presence of e gene indicates infection with sarbecovirus (b-βcov) but
absence of sars-cov-2
3
both e gene and rdrp and n gene
detected both e gene and rdrp and n gene detected
page 2
sr. No. Result comment
4
e gene not detected but rdrp and n gene detected
presence of sars- cov-2 (n or/and rdrp) specific rna indicates
infection with covid-19.
5 internal control not detected inconclusive, rtpcr failure due to possible presence of inhibitors.
Repeat sample is requested
test attributes and limitations:
1. Samples must be received at the laboratory under appropriate conditions within 48 hrs of collection to ensure preservation of viral rna.
2. Negative result does not rule out the possibility of cavity -19 infection. Presence of inhibitors, mutations & insufficient organism rna can
influence the result. False negative results may be seen in samples collected too early or too late in the clinical course of the infection. Kindly refer to the
latest icmr guidelines.
3. A single negative test result, particularly if this is from an upper respiratory tract specimen, does not exclude infection. A positive alternate pathogen
does not necessarily rule out covid-19 infection, as little is yet known about the role of coinfections.
4. Repeat sampling and testing of lower respiratory specimen is strongly recommended in severe or progressive disease. The repeat specimens may be
considered after a gap of 2 - 4 days after the collection of the first specimen for additional testing if required.
Patient instructions:
1. Kindly consult referring physician/ authorized govt. Hospital for appropriate follow up.
2. Details of all the positive patients will be communicated to epidemiology cell whom you are requested to support.
3. “positive” status needs to be notified to the appropriate authorities as per the existing rules/regulations, while we shall also be doing the same.

Dr.Hara Prasad Mishra · Accepted Answer

Hello
dear, sorry to know about ill health.
Your mother is covid negative. The report only suggests different biological and clinical interpretations on the basis of different values and various gene positivity. Hope I have briefly answered your query. Kindly do not self medicate, as it might be harmful for you.
Take a balanced diet and adequate fluid intake.
Practice relaxation and meditation techniques.
In case you have more doubts or queries, please book a private / online consultation (hp0d9a is my code) so that we can discuss in detail your problems and you can feel relieved after finding your answers. Medicine can not be advised for open question, for medicine contact on private consultation.
Best wishes
stay safe stay healthy.

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