PCR for MTB (Tissue) Test

Polymerase Chain Reaction aka PCR is one of the most promising test to diagnose TB. Tuberculosis is an infectious disease caused by bacteria named Mycobacterium tuberculosis (MTB). PCR is a simple and rapid test used for detection of Mycobacterium tuberculosis because it amplifies a DNA sequence which is unique to mycobacteria. In PCR, short sections of DNA are converted into large copies in a process known as amplifying of DNA. It enables specific genes of interest to be detected or measured. After the results of the test, a doctor is able to diagnose and prescribe a patient with medication for TB which in turn aids any irreversible damage.

There is no special preparation required before the test. It is recommended that you wear loose fitting clothes for the test. Wearing sleeveless or short-sleeved shirt should be preferred.

You are not required to change or alter your diet. Drinking water and staying hydrated is highly encouraged before the test as it helps in drawing the blood easily and with no or little pain. Ask your lab provider or doctor beforehand, if you are required to fast or not and any other instructions to be followed during the test especially if you are taking any medication for TB.

Few millilitres of blood would be obtained from your body. The entire procedure will not take more than
15minutes or so. A well-trained phlebotomist will assist you in the entire process.

• A tight band is put on the arm to slow the blood flow and causing the vein to swell.

• An antiseptic wipe or cotton dipped in alcohol is used to clean the area of skin.

• A needle is then injected through the rinsed area into your vein and a blood sample is drawn.

• The blood is saved in a container and labelled with your name and details.

• This sample is carried to the research laboratory for further examination.