Anti Smith Antibody Test

Antibody tests are blood tests that determine specific antibodies which simplify the diagnosis of lupus, Antinuclear antibody (ANA) Anti-RNP. Anti-Smith (Sm),.Anti-Smith antibodies are present in some cases of systemic lupus erythematosus (SLE) and mixed connective-tissue disease (MCTD). Smith antigens are elements of the extractable nuclear antigens (ENAs); typically they are the proteins that are resistant to ribonuclease.

An enzyme-linked immunosorbent assay, also named ELISA or EIA, is the test that detects and measures antibodies in your blood. On the day of the test, you may to have wear a T-shirt or short-sleeved shirt to let easier contact while drawing the blood.

it can also be useful in confirming a diagnosis of systemic lupus. Contrasting anti-dsDNA, anti-Sm does not associate with the presence of kidney lupus. They are connected with central nervous system involvement, kidney disease, lung fibrosis and pericarditis in SLE, but they are not related with disease activity.

Serum from the blood sample is then added to a microscopic slide prepared with specific cells on the slide surface. If the patient has antinuclear antibodies the serum will bind to the cells on the slide. Subsequently, a second antibody tagged with a fluorescent dye is added so that it joins to the serum antibodies and cells that have bound together. Lastly the slide is viewed using a fluorescence microscope.