Seeds within the hard shell are produced by a small tree called pistachio plant. People eat these seeds as food. 20% of proteins are entailed in it. Among these proteins, some of the individual proteins are responsible for allergic reactions. The symptoms of these reactions are swelling, itchiness and skin rush. If the condition becomes serious then it leads to cardiovascular or gastrointestinal problems. These problems can affect our life harshly. The test which is done to diagnose the allergic reactions caused by consumption of pistachio is termed as Allergen, individual-food pistachio test.