Through Dengue-Antigen-NS1, diagnoses of acute DV infection can be seen. The Dengue antigen NS 1 is detected within 24 hours and lasts up to 9 days. Dengue-Antigen-NS1 is done to diagnose the exposure to acute infection and dengue virus. The method of detection is called enzyme-linked immunosorbent assay. A doctor can order for Dengue-Antigen-NS1 testing if the patient is suspected of acute dengue illness. If the doctor finds the fever persists for more than six days, the test conduction becomes a must. This can also involve symptoms of musculoskeletal pain, headaches, retro-orbital pain or transient macular rash. These symptoms are seen more in children.