Nicotine test is often done to assess tobacco use. It can be analysed both qualitatively and quantitatively. Qualitative analysis is done to find the presence or absence of nicotine, whereas quantitative analysis is done to find the concentration of the substance present. A urine or blood nicotine test is usually done when the patient experiences an overdose of nicotine. Long-term use of or exposure to nicotine can lead to heart disease, COPD, lung cancer, respiratory infections and stroke. It can also trigger asthma and spur blood clot formation.