FISH, 1p and 19q codeletion, Brain Cancer Test

Fluorescent in situ hybridization (FISH) is a steadfast, economical technique that is capable of detecting the abnormality in minimal amounts of tumor cells on formalin-fixed, paraffin-embedded (FFPE) tissue sections. 1p/19q codeletion is a constructive prognostic marker for oligodendroglial tumors .The test compares result in tumors with simple deletions of 1p or 19q to those with relative deletions defined as the presence of both increased copy number (polysomy) and 1p/19q codeletion.

Patients need to submit a formalin-fixed, paraffin-embedded (FFPE) tumor tissue block. Blocks arranged with substitute fixation methods is suitable; provide fixation method used.

This test is used as an aid in diagnosing Oligodendroglioma tumors and diagnose the response to therapy. It is typically supportive in tumors with intricate hybrid morphology which necessitates demarcation from pure Astrocytomas to aid the occurrence of oligodendroglial Differentiation/lineage. The test is usually suggested in mixed Oligoastrocytomas. It is also suggested when a diagnosis of Oligodendroglioma, including low-grade World Health Organization (WHO, grade II) and anaplastic (WHO, grade III) is rendered.

This test does not include a pathology consult. If a pathology consultation is suggested, the test should be conducted and the appropriate FISH test will be ordered and performed. This test comprises first probe set (2 FISH probes) and expert interpretation of results.