Biotidinase Enzyme (Quantitative) Test

The enzyme which is created by BTD gene is termed as Biotinidase. We cannot separate vitamin biotin from the food without biotinidase activity and thus the body cannot use it. Biotin can be recycled from the enzymes which play a significant role in metabolism by biotinidase enzyme. Certain fats, carbohydrates or proteins are not processed by specific enzymes known as carboxylases due to deficiency of biotinidase. The test which conducted to examine deficiency of biotinidase is termed as Biotinidase Enzyme (Quantitative) test.

Preparation
We have to make some preparations before carrying out the biotinidase enzyme (quantitative) test. Following are the preparations.
• We should consult with the doctor if we are taking any medical treatment.
• We should consult with the doctor if we are suffering from any type of allergy.
• We should be calm (if we are afraid of the pain caused due to insertion of the needle) while taking the blood sample from our body. As, fast breathing will affect the accuracy of the test.

Biotinidase enzyme (quantitative) test proves to be useful. Following are the uses of this test.
• It is used to determine the deficiency of biotinidase enzyme.
• We will not face the serious condition if this test is followed on time.
• It will protect us to suffer from weak muscle tone, seizures, hearing and vision loss, breathing problems, hair loss and skin rashes.

In this test, the blood sample is taken by inserting needle from the searched vein of the hand. On filter paper, as an assay medium, the blood sample is allowed to be deposited on filter paper. This can be done if, on the standard sample collection paper, we predeposit N-biotinyl-p-aminobenzoic acid. A simple extraction step from a dried blood spot is the requirement of assay mixture analysis. Quantification of the product by LC-MS follows this extraction step.