Amyloid Stain Test

Amyloid Stain test involves a simple and relatively simple approach to the diagnosis of systemic amyloidosis and is positive test with systemic amyloidosis. If the test is negative, then there is a suspicion of disease being present, a biopsy of an involved organ system for instance heart, kidney, or liver may need to be done to make the diagnosis.

No special preparation is needed for Amyloid Stain test. You need to inform your doctor if you are taking any other medications or have any prior medical situation or allergies before if you undergo Amyloid Stain test. Your doctor depending on your condition will give specific instructions.

The Amyloid test is used to find out any abnormal, fibrous, extracellular proteinaceous deposits found in organs such as liver, kidneys, spleen etc. This condition of deposition of amyloid in tissues is known as Amyloidosis.

The tissue is deparaffinized and sectioned in distilled water. Then it is stained in congo red working solution for 10 minutes later rinsed in distilled water and following which it is differentiated quickly (9-10 dips) in alkaline alcohol solution. Later, it is rinsed in tap water.Counterstained with Meyer’s Hematoxylin for 30 seconds. It is dipped in ammonia water for 30 seconds until sections turn blue. Rinse in distilled water for 10 minutes. Dehydrate, clear and mount.