Paroxysmal Nocturnal Hemoglobinuria (PNH) is a rare acquired stem cell disorder which is associated with periodic hemolytic events. This remediable clonal condition has been associated with thrombosis and cytopenias and is caused by the abnormal X-linked phosphatidylinositol glycan class A gene. In recent improvements in PNH diagnostics, the flow cytometry (FCM) can detect PNH cells ab about 0.01% of the total cells. Also, limitations of fluorescent inactivated aerolysin (FLAER) has been recognized for the measurement of the RBC clone. PHN confirmation test is done on a blood sample to detect and measure the level of Paroxysmal Nocturnal Hemoglobinuria in the blood. The PNH with FLAER flow cytometry quantifies GPI-AP-deficient blood cells by using a variety of GPI-AP markers.
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