HPV Realtime PCR- Qualitative Test

A real-time polymerase chain reaction (Real-Time PCR) is a laboratory technique of molecular biology. It is also known as quantitative polymerase chain reaction. The name has been given as it is based on the polymerase chain reaction (PCR). The changes in The PCR process take place for approx 25 – 50 times. It is by recurring nature. The temperatures and the timings used for each cycle depend on a wide variety of parameters. There are two ways used commonly. It is done basically to detect PCR products in real- time. The acronym "RT-PCR" denotes reverse transcription polymerase chain reaction.

Human papillomavirus (HPV) increase the sensitivity. It takes place in vitro. The thermal cycler is well- known to rapidly heat and chill samples. The cycles take place on three levels the first, at around 95 °C, the second, at a 50-60 °C, the third, at between 68 - 72 °C. It uses the physicochemical properties of the nucleic acids and DNA polymerase. It is generally smaller in size and thus it takes utmost advantage. They are able to increase their number. Sometimes, in four steps PCR the fluorescence is also measured. It lasts for few seconds only. But it gives an absolute result.

It is useful in many aspects.

The Abbott RealTime High Risk (HR) HPV assay is a qualitative aspect. Many labs even try to find out solutions for low, medium, and high. Detection of single and mixed infections is the primary step. High risk HPV genotypes: 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 are measured and studied. It is ensured that the homogeneous target amplification is utilized. Collection in liquid cytology media is done then.